Deepak Nair together with Muthukumaran Venkatachalapathy of the Indian Institute of Science, Bangalore, have just reported the use of The Twinkle Factory‘s FAST for super resolution imaging in live cell.
See in Nanoscale: Live cell super resolution imaging by radial fluctuations using fluorogen binding tags. Nanoscale, 2019, 11, 3626-3632.
Fluorescence-Activating and absorption-Shifting Tag (FAST) is a novel genetically encoded optical highlighter probe. Since the fluorescence of FAST originates from the stochastic and reversible diffusive association of a fluorogenic ligand, we investigate the application of FAST using Super-Resolution Radial Fluctuations (SRRF) to achieve routine imaging below the diffraction limit in a widefield epifluorescence microscope. We show that intensity fluctuation analysis like SRRF allows the imaging of FAST-tagged proteins with sub – 100 nm resolution in live cells. FAST co-labeled with conventional fluorophores enables real time multicolour 2D and 3D super-resolution imaging, indicating that FAST can be used for the observation of sub-diffraction limited structures in both living and fixed samples