How to order TFFluorogens

Plasmids for FAST and splitFAST in mammalian cells are now available on Addgene.

Step 1: Request for quotation

This is your first order?  Or you want to order quantities larger than 1,000 nmol?  Ask a quote to Charlotte or Luc by e-mail: contact@thetwinklefactory.com

All TFFluorogens are available in three formats: 250, 500 and 1,000 nmol.

Quantity Price
250 nmol 190 €
500 nmol 350 €
1,000 nmol 600 €

a) We offer a 50% introductory discount for first-time orders above 2 x 250 nmol.

b) We charge handling and shipment fees for orders below 1,000 €: 30 € for shipments into Europe, 50 € overseas.

Your request for quotation should display the following details besides quality and amount:  Your name / your institution – firm / billing address / shipping address (including a telephone # when relevant)

Within two days we will provide you with a quote.

Step 2: Order

Upon reception of our quotation, or directly if you are a registered customer, can you order. There are two options:

1/ You prefer to pay by bank wire onto our bank account:  issue a purchase order and send it by e-mail to Charlotte or Luc,

2/ You prefer to pay by credit card (we welcome Mastercard or Visa, no Amex, nor Diners):  upon request, we will provide you with a secure link to our bank back office

Step 3: Shipment

We’ll send you within 10 days the products ordered.  They will come with an invoice and a delivery note.  Both will also come by e-mail upon shipment.

TFFluorogens

TFLime – 480541-250 

To be used with: FAST1 (FAST1 sequence), FAST2 (FAST2 sequence), tdFAST1 (tdFAST1 sequence) or tdFAST2 (tdFAST2 sequence)
Absorption and emission wavelengths when bound to
    FAST2: 480 nm / 541 nm – FAST1: 481 nm / 540 nm
Molar absorption coefficient when bound to
    FAST2: 41,000 M–1cm–1 – FAST1: 45,000 M–1cm–1 
Fluorescence quantum yield when bound to
    FAST2: 23 % – FAST1: 23 %
Affinity constant at 25°C of its complex with
    FAST2: 0.07 µM  – FAST1: 0.13 µM
Download TFLime Datasheet

TFAmber – 499558-250

To be used with: FAST1 (FAST1 sequence), FAST2 (FAST2 sequence), tdFAST1 (tdFAST1 sequence) or tdFAST2 (tdFAST2 sequence)
Absorption and emission wavelengths when bound to
    FAST2: 499 nm / 558 nm – FAST1: 499 nm / 562 nm
Molar absorption coefficient when bound to
    FAST2: 46,000 M–1cm–1 – FAST1: 48,000 M–1cm–1 
Fluorescence quantum yield when bound to
    FAST2: 57 % – FAST1: 49 %
Affinity constant at 25°C of its complex with
    FAST2: 0.06 µM  – FAST1: 0.08 µM
Download TFAmber Datasheet

TFCitrus – 495560-250

To be used with: FAST1 (FAST1 sequence), FAST2 (FAST2 sequence), tdFAST1 (tdFAST1 sequence) or tdFAST2 (tdFAST2 sequence)
Absorption and emission wavelengths when bound to
    FAST2: 495 nm / 560 nm – FAST1: 494 nm / 561 nm
Molar absorption coefficient when bound to
    FAST2: 39,000 M–1cm–1 – FAST1: 40,000 M–1cm–1 
Fluorescence quantum yield when bound to
    FAST2: 49 % – FAST1: 36 %
Affinity constant at 25°C of its complex with
    FAST2: 0.20 µM  – FAST1: 0.31 µM
Download TFCitrus Datasheet

TFCoral – 516600-250

To be used with: FAST1 (FAST1 sequence), FAST2 (FAST2 sequence), tdFAST1 (tdFAST1 sequence) or tdFAST2 (tdFAST2 sequence)
Absorption and emission wavelengths when bound to
    FAST2: 516 nm / 600 nm – FAST1: 518 nm / 600 nm
Molar absorption coefficient when bound to
    FAST2: 38,000 M–1cm–1 – FAST1: 39,000 M–1cm–1 
Fluorescence quantum yield when bound to
    FAST2: 40 % – FAST1: 31 %
Affinity constant at 25°C of its complex with
    FAST2: 0.41 µM  – FAST1: 0.97 µM
Download TFCoral Datasheet

TFAmber-NP – 505559-250

To be used with: FAST1 (FAST1 sequence) or tdFAST1 (tdFAST1 sequence
Absorption and emission wavelengths
when bound to
    FAST1: 505 nm / 559 nm
Molar absorption coefficient when bound to
    FAST1: 61,000 M–1cm–1 
Fluorescence quantum yield when bound to
    FAST1: 8 %
Affinity constant at 25°C of its complex with
    FAST1: 1.3 µM
Download TFAmber-NP Datasheet

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